目的 利用AB-8大孔树脂结合醇沉法对紫色马铃薯花色苷的纯化工艺进行研究。方法 以River John Blue 基因型紫色马铃薯为原料,以浸提的方法得到紫色马铃薯花色苷粗提物,然后分别从静态吸附的角度(搅拌、上样浓度、上样pH、洗脱液浓度、洗脱液pH)和动态吸附的角度(上样流速和洗脱流速)对粗提物进行了纯化工艺的研究,以吸附率、解析率和色价值作为评价指标,在此单因素实验基础上,进行正交试验分析得到最后的优化工艺条件。结果 AB-8型大孔树脂的最佳纯化条件为:搅拌,上样浓度为0.500 mg·mL-1,上样pH为2,洗脱液乙醇体积分数为70%,洗脱液pH为1,上柱吸附流速为1 mL·min-1,洗脱流速为1 mL·min-1。在此工艺条件下,花色苷的色价值可高达75.40,比纯化前提高了8.43倍。结论 此纯化工艺的方法可行性高,提高了花色苷的纯度及色价,为紫色马铃薯花色苷的市场开发及应用奠定了一定的实验基础。
Abstract
OBJECTIVE To study the purification process of anthocyanins from purple Solanum tuberosum by AB-8 macroporous resin combined with alcohol precipitation method. METHODS Using River John Blue genotype purple Solanum tuberosum as raw material, purple Solanum tuberosum anthocyanin crude extract was obtained by leaching method. Then, the purification process of the crude extract was studied by using static adsorption (stirring, loading concentration, loading pH, eluent concentration, eluent pH) and dynamic adsorption (loading flow rate and elution flow rate), respectively, taking adsorption rate, resolution rate and color value as the evaluation indexes. On the basis of the single factor experiment, orthogonal optimization analysis was carried out to get the final optimized process condition. RESULTS The optimum conditions for the purification of AB-8 macroporous resin were as follows stirring, loading concentration 0.500 mg·mL-1, loading pH 2, ethanol concentration 70% and elution pH 1. The column flow rate was 1 mL·min-1, and the elution flow rate was 1 mL·min-1. Under this condition, the color value of anthocyanin was able to reach 75.40, which was 8.43 times higher than that before purification. CONCLUSION The purification method has high feasibility and can improve the purity and color value of anthocyanins, which laids a foundation for the market development and application of anthocyanins of purple Solanum tuberosum.
关键词
紫色马铃薯 /
花色苷 /
大孔树脂 /
纯化工艺
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Key words
purple Solanum tuberosum /
anthocyanin /
macroporous resin /
purification process
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中图分类号：
R284
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参考文献
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脚注
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基金
生物多肽糖尿病药物湖北省协同创新中心基金资助(鄂教科函[2012]56号);湖北省生物多肽糖尿病药物工程技术研究中心基金资助(鄂科技通[2014]72号)
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